Brice Moukengue
PhD in Biology
36 years old
Driving License
Loubomo Congo - Brazzaville
Professional Status
Employed
Available
About Me
I am a PhD from the university of Nantes. I have no problem changing locations, and I can be available rapidly.
PhD student in Biology
PHY-OS Bone sarcoma and remodeling of calcified tissues (INSERM UMR 1238 )
October 2016
to November 2019
- Using new drugs and RNA interference (si/shRNA) to target cellular stress proteins in bone cancer (Osteosarcoma)
- Evaluating the effects of gene inhibition on cell processes (proliferation, death, migration, drug resistance...)
- Working directly with bioinformaticians to find new therapeutic targets in bone cancer (high throughput experiments : RNA-sequencing, ChIP-sequencing, RPPA and RIME)
- Studying the effects of gene inhibition on tumor growth in murine models (tumor cells injection, tumor volume monitoring, sacrifice, tumor characterization by immunohistochemistry)
- Wrote an article (first author) on the inhibition of the MTH1 (NUDT1) enzyme in Osteosarcoma, and its therapeutic effects
- Wrote a chapter in a published book, a protocole describing the good practice for gene expression analysis (from RNA extraction to primer design and qPCR)
- First author in an yet unpublished study on the transcriptionnal network of the HSF1 trascription factor in Osteosarcoma
Histology teacher (2nd year medical students)
University of Nantes
2016
to 2018
- Teaching Histology to 2nd year medical students at the university of Nantes
Cleaner
APC propreté
Since January 2020
- Cleaning professional spaces (Schools, laboratories, medical offices, industry)
Trainee Laboratoire de Physiopathologie de la Résorption Osseuse (LPRO)
Laboratoire de Physiopathologie de la Résorption Osseuse (INSERM UMR 957)
November 2013
to May 2016
Internship
Nantes
France
- Osteosarcoma and Ewing sarcoma cells culture
- Cell viability analysis (tetrazolium salts, crystal violet and cell count)
- Cell cycle analysis by flow cytometry
- Plasmidic DNA, RNA and protein extraction
- Western blot, RT-qPCR,
- Inhibition/overexpression of specific genes (RNA interference/plasmid)
- Lentiviral particles production
Detailed Description
- The study of the effects of new drugs on sarcoma cells
Cleaner
HELP
October 2014
to November 2015
Part-time
Nantes
France
- Washing dishes
- Laundry
- Floor cleaning
- Cleaning the common area of a residential building
Volunteer for Bethlehem 44
Bethlehem 44
July 2012
to September 2015
Volunteer Work
Nantes
France
- Food packaging and transportation
- Food selling
- Cardboard pickup and transportation to a recycling center (Véolia)
Company website
Building worker
Private individual
June 2006
to July 2006
Temporary Work
Pointe-Noire
Congo - Brazzaville
- Building materials and tools' transportation
- Tools' maintenance
- Cleaning of the construction site
