Brice Moukengue
PhD in Biology
36 years old
Driving License
Loubomo Congo - Brazzaville
Professional Status
Employed
Available
About Me
I am a PhD from the university of Nantes. I have no problem changing locations, and I can be available rapidly.
PhD student in Biology
PHY-OS Bone sarcoma and remodeling of calcified tissues (INSERM UMR 1238 )
October 2016
to November 2019
- Using new drugs and RNA interference (si/shRNA) to target cellular stress proteins in bone cancer (Osteosarcoma)
- Evaluating the effects of gene inhibition on cell processes (proliferation, death, migration, drug resistance...)
- Working directly with bioinformaticians to find new therapeutic targets in bone cancer (high throughput experiments : RNA-sequencing, ChIP-sequencing, RPPA and RIME)
- Studying the effects of gene inhibition on tumor growth in murine models (tumor cells injection, tumor volume monitoring, sacrifice, tumor characterization by immunohistochemistry)
- Wrote an article (first author) on the inhibition of the MTH1 (NUDT1) enzyme in Osteosarcoma, and its therapeutic effects
- Wrote a chapter in a published book, a protocole describing the good practice for gene expression analysis (from RNA extraction to primer design and qPCR)
- First author in an yet unpublished study on the transcriptionnal network of the HSF1 trascription factor in Osteosarcoma
Histology teacher (2nd year medical students)
University of Nantes
2016
to 2018
- Teaching Histology to 2nd year medical students at the university of Nantes
Cleaner
APC propreté
Since January 2020
- Cleaning professional spaces (Schools, laboratories, medical offices, industry)
2nd year Master degree in Biology, Biotechnology and therapeutic research
Sciences and techniques faculty of Nantes
September 2015
to May 2016
Extensive training in cellular and molecular biology and pharmacology, applied to humans and animals' diseases
Skills
Technical skills
- Lentiviral particles production in a L3 laboratory
- Diploma in animal experimentation (rabbit and rodents)
- Cell culture and viability assay (Crystal violet or tetrazolium salts)
- Flow cytometry (study of apoptosis by annexin V antibody and cell cycle)
- Immunohistochemistry and immunofluorescence for cell characterization
- Overexpression or inhibition of a specific gene by transfection or transduction (plasmide, si/shRNA)
- Plasmidic DNA, RNA and protein extraction
- Gene expression analysis (western blot, reverse transcription, primer design and qPCR)
- Study of DNA damage by comet assay
- Protein interaction assays (Immunoprecipitation, proximity ligation assay)
- Chromatin immunoprecipitation
- Cell migration and invasion assay using boyden chambers
Transversal skills
- Communication skills : Two prizes for best oral presentation at the JFBTM 2017 convention and best poster presentation at JFBTM 2019. Multiple oral and poster presentaions at national and international scientific conventions (Congress of translational research in oncopediatry (2018), CTOS congress in Roma (2018), Canceropole grand-Ouest convention (2017 & 2018), BBC meeting (2017) and others
- Ability to teach and supervise trainees (taught histology to 2nd year medical students for 3 years and supervised several interns (licence, master and medical interns))
- Ability to work collaboratively with colleagues from different backgrounds (scientists, Medical Doctors, bioinformaticians, students...) and explain technical concepts clearly
- Ability to choose and use the suitable statistical test for the interpretation of experimental results
- Can write reports, summaries and protocols in French or English (Wrote a chapter for a published book of protocols in bone cancer study)
Langages
- French (native langage)
- English read, written and spoken (TOEIC score : 980/990)
