Brice Moukengue
PhD in Biology
36 years old
Driving License
Loubomo Congo - Brazzaville
Professional Status
Employed
Available
About Me
I am a PhD from the university of Nantes. I have no problem changing locations, and I can be available rapidly.
Skills
Technical skills
- Lentiviral particles production in a L3 laboratory
- Diploma in animal experimentation (rabbit and rodents)
- Cell culture and viability assay (Crystal violet or tetrazolium salts)
- Flow cytometry (study of apoptosis by annexin V antibody and cell cycle)
- Immunohistochemistry and immunofluorescence for cell characterization
- Overexpression or inhibition of a specific gene by transfection or transduction (plasmide, si/shRNA)
- Plasmidic DNA, RNA and protein extraction
- Gene expression analysis (western blot, reverse transcription, primer design and qPCR)
- Study of DNA damage by comet assay
- Protein interaction assays (Immunoprecipitation, proximity ligation assay)
- Chromatin immunoprecipitation
- Cell migration and invasion assay using boyden chambers
Transversal skills
- Communication skills : Two prizes for best oral presentation at the JFBTM 2017 convention and best poster presentation at JFBTM 2019. Multiple oral and poster presentaions at national and international scientific conventions (Congress of translational research in oncopediatry (2018), CTOS congress in Roma (2018), Canceropole grand-Ouest convention (2017 & 2018), BBC meeting (2017) and others
- Ability to teach and supervise trainees (taught histology to 2nd year medical students for 3 years and supervised several interns (licence, master and medical interns))
- Ability to work collaboratively with colleagues from different backgrounds (scientists, Medical Doctors, bioinformaticians, students...) and explain technical concepts clearly
- Ability to choose and use the suitable statistical test for the interpretation of experimental results
- Can write reports, summaries and protocols in French or English (Wrote a chapter for a published book of protocols in bone cancer study)
Langages
- French (native langage)
- English read, written and spoken (TOEIC score : 980/990)
Computing
- Advanced use of word processing, presentation and spreadsheets softwares (office suite among others)
- Advance use of GraphPad PRISM
- basic HTML and Javascript knowledge
