Compétences — Nicolas Sprynski

Compétences

"Galleria mellonella as an infection model for selected agents"
N. SPRYNSKI, E. Valade, F. Neulat-Ripoll. Methods in Molecular Biology. 2014;1197:3-9.
"Galectin-1 enhances Brucella virulence through interaction with type IV secretion protein VirB5"
N. SPRYNSKI, F. Bard, A. Anginot, F. Poirier, G. Rabinovich, C. Schiff, D. O’Callaghan, A. C. Vergunst. PNAS in revision
"Complementation of a Brucella virB5 mutant is affected by VirB5 protein levels and multiple virB promoter copies"
N. SPRYNSKI, C. Felix, D. O’Callaghan, A. C. Vergunst. FEBS Open Bio Volume 2, 2012, Pages 71–75

Biochimie:
- PCR
- Clonage,
- Production et Purification de protéines,
- WesternBlot et électrophorèse SDS-PAGE.
Interactions protéiques:
- Double hybride,
- GST pull down,
- BiFC (Bimolecular fluorescence complementation).
- DARTS (Drug affinity responsive target affinity)
Culture cellulaire :
- Entretien de lignées
- Différenciation et culture de
  macrophages primaires issus de moelle osseuse
- Transfection transitoire et stable
Infections bactériennes
Microscopie : Immunofluorescence
Travail en laboratoire confiné NSB3
Microbiologie:
- Tests phénotypiques
- Biofilm formation
- MIC / MBEC

"Développement d'un modèle alternatif chez les bactéries de la menace"
N. SPRYNSKI*, F. Neulat-Ripoll*(orateur), F. Biot, E. Valade. Congrés de la Société Francaise de Microbiologie Lille Fév. 2013
"Multidrug Resistance and Expression of efflux pumps in chloramphenicol-selected strains of Burkholderia pseudomallei and Burkholderia thailandensis"
Biot F., Thibault F., SPRYNSKI N., Neulat-Ripoll F., Vidal D., Pagès JM., Valade E., Medical Biodefense Conference, Munich (Allemagne) Oct. 2011
"Résistance induite par la doxycycline : coopération entre les pompes d’efflux chez les Burkholderia"
Biot F., Thibault F., SPRYNSKI N., Neulat-Ripoll F., Vidal D., Pagès JM., Valade E., Journée des Thésards - La Tronche, Juin 2011
"Galectin-1 enhances Brucella virulence through interaction with type IV secretion protein VirB5"
N. SPRYNSKI, F. Bard, A. Anginot, F. Poirier, G. Rabinovich, C. Schiff, D. O’Callaghan, A. C. Vergunst. 1er Séminaire du département de Microbiologie, IRBA, La Tronche Fév. 2011.
"Galectin-1 enhances Brucella virulence through interaction with type IV secretion protein VirB5"
N. SPRYNSKI, F. Bard, A. Anginot, F. Poirier, G. Rabinovich, C. Schiff, D. O’Callaghan, A. C. Vergunst (orateur). Annual Brucellosis Research Conference, Chicago, USA. Déc. 2009.
"Interactions between the Brucella suis VirB pilus and host proteins"
N. SPRYNSKI, D. O’Callaghan, A. C. Vergunst. COST Action 845. Brucellosis in animals and man, Italy, Oct, 2006.

"Role of the active efflux in doxycycline selected strains of Burkholderia thailandensis"
Biot F., Neulat-Ripoll F., SPRYNSKI N., Thibault F., Vidal D., Pagès J.-M., Valade E., European Melioidosis Network meeting. Amsterdam, Pays-Bas. Fév. 2012.
"QseC gene in Francisella tularensis: a prime target for the development of vaccine strain against tularaemia"
Mokrievitch N., Kondakova A, SPRYNSKI N., Pavlov V., Valade E 7th International conference on tularemia, Breckenridge Colorado, USA. Sept. 2012
"Analysis of the involvement of Gal-1 in Brucella infection"
N. SPRYNSKI, D. O’Callaghan, A. C. Vergunst. Workshop "Signal Transduction in Host-Bacterial Interactions", Jerusalem, Israel. Oct. 2007.

Nicolas Sprynski