Sophie Rolati
Assistante de Recherche
Driving License
Miami United States (Florida)
Employed
Available
Research Associate
University of Miami
Since April 2011
Miami
United States - Florida
- Characterization and validation of genetic markers associated with Alzheimer’s disease (AD) and Age-related Macular Degeneration (AMD)
- Whole Exome Sequencing project on approximatively 3300 African-American samples on Illumina instrument. o In charge of samples selection and allocation from Biorepository, quality control, quantitation.
- RNA sequencing on 500 cases and controls AD Caucasian and African American samples. In charge of RNA extraction from PAXgene blood tubes using Qiagen automated instrument (QIAsymphony), samples quality control with Nanodrop, Agilent, Bioanalyzer and Tapestation.
Research Associate
French Alternative Energies and Atomic Energy Commission (CEA)
April 2009
to October 2010
Grenoble
France
- Optimization of semi-automated cell isolation and RNA extraction from whole blood sample
- Manual and automated cell isolation and DNA/RNA extraction from whole blood sample
- Specific and generic nucleic acid isolation on magnetic beads and microsystems
Research Associate
bioMérieux
January 2008
to December 2018
- Developed and optimized sample preparation protocols for integration in high-density DNA chip.
- Validation of manual and automated RNA extraction from PAXgene samples for gene expression analysis on Affymetrix chips.
- Collaborate with laboratories as University of Pennsylvania, National Cell Repository for Alzheimer's Disease (NCRAD), Vanderbilt, Mayo Clinic
- Interact with analysts, clinicians, post-doctoral researchers and students
- Report preparation to be presented to the Laboratory Director
- Poster presentation during international conferences
- Prioritize and accomplish tasks, while meeting given deadlines.
- Project management and tracking for AD and AMD research study
- Efficiently train and supervise personnel and 3 to 4 students per year on laboratory techniques
- Evaluate prospective Associate applicants by participating in interviews as needed.
- Manual and automated extraction from whole blood or tissue samples
- RNA sequencing on 500 cases and controls AD Caucasian and African American samples
- Genotype significant variants found through whole exome sequencing in collaborator cohort within large African American dataset to look for replication
- Validation of whole genome association study data
- Comparison of results within and between dataset, submission to database
- Sanger sequencing on all samples ascertained within the AD study for markers of interest
